A plant virus protein, NIa-pro, interacts with Indole-3-acetic acid-amido synthetase, whose levels positively correlate with disease severity

A plant virus protein, NIa-pro, interacts with Indole-3-acetic acid-amido synthetase, whose levels positively correlate with disease severity

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Potato virus Y (PVY) is Hoodies an economically important plant pathogen that reduces the productivity of several host plants.To develop PVY-resistant cultivars, it is essential to identify the plant-PVY interactome and decipher the biological significance of those molecular interactions.We performed a yeast two-hybrid (Y2H) screen of Nicotiana benthamiana cDNA library using PVY-encoded NIa-pro as the bait.The N.

benthamiana Indole-3-acetic acid-amido synthetase (IAAS) was identified as an interactor of NIa-pro protein.The interaction was confirmed via targeted Y2H and bimolecular fluorescence complementation (BiFC) assays.NIa-pro interacts with IAAS protein and consequently increasing the stability of IAAS protein.Also, the subcellular localization of both NIa-pro and IAAS protein in the nucleus and cytosol was demonstrated.

By converting free IAA (active form) to conjugated IAA (inactive form), IAAS plays a crucial regulatory role Fabric Chalk in auxin signaling.Transient silencing of IAAS in N.benthamiana plants reduced the PVY-mediated symptom induction and virus accumulation.Conversely, overexpression of IAAS enhanced symptom induction and virus accumulation in infected plants.

In addition, the expression of auxin-responsive genes was found to be downregulated during PVY infection.Our findings demonstrate that PVY NIa-pro protein potentially promotes disease development via modulating auxin homeostasis.